WHY YOU SHOULD CHOOSE INVIVO!
Benefit from InVivo´s expertise. InVivo has invested several years into research to gain the best results for your project.
Rely on us for your outsourcing needs!
From sequence to product within weeks.
With our efficient method of transient gene expression, it is possible to obtain mammalian derived proteins within weeks. Stable cell line development, which is always related to hard and time-consuming lab work, is no longer needed.
TRANSIENT GENE EXPRESSION OF RECOMBINANT ANTIBODIES
Thanks to our expertise, we guarantee 40 mg of your recombinant antibody (IgG) in high quality within weeks. Try our sequence to antibody service.
- 40 mg recombinant antibody,
- QC guarantee of ≥ 90% purity
=> For € 4,000 (without DNA-synthesis)
We only need the sequence of the variable domains and the requested subclass of your antibody and we can start right away. If we do not meet our guarantees, you don’t have to pay for the production steps. Only the costs for the DNA synthesis have to be covered. The synthesis vector can be provided.
We use HEK cells to produce your antibody and purify it with protein-A affinity chromatography. You´ll receive your antibody 5 weeks after DNA synthesis. Get your recombinant antibody in quick time at reasonable costs! InVivo’s reliable service!
If the sequences are not available we are able to sequence the variable domains from your hybridoma cells. Please contact us, we will be pleased to advise you.
FEASIBILITY STUDY FOR RECOMBINANT PROTEINS
Use our feasibility study to start up your recombinant protein projects. HEK cells are used at InVivo for transient expression. The ease of handling, the good growth rates, excellent transfections rates and high productivity promises satisfactory results.
The feasibility study includes:
- Plasmid preparation
- Mammalian cell transfection
- Transient expression in small-scale
- One-step purification of supernatant using an affinity chromatography (HIS-Tag, Fc, etc.)
- QC: SDS-PAGE to estimate purity, A280 value
With the help of the feasibility study, we evaluate the expression of your protein, undertake test purification and check the quality. This enables us to calculate prices for up-scaling of future production. Tags for purification are highly recommended, as they simplify purification processes.
This service costs € 2,000. Please note that DNA-synthesis is not included in this offer, as we outsource this service.
Profit from our powerful and productive protein expression method!
For target discovery and lead identification, we can run up to 100 expressions on a small-scale in parallel. Use this multiplicity for your variety!
Large-scale production of recombinant antibodies and recombinant proteins in gram-scale is possible with our enhanced transfection method. Cell lines, vector design, plasmid preparation, transfection reagents, transfection media and cultivation process are all systematically boosted for the maximum protein output.
Cultivation can be done in shake flasks or stirred bioreactor systems of up to 100 L depending on your needs.
InVivo offers the service of plasmid preparation (200 µg – 1g) suitable for transfection of mammalian cell lines.
EVERY PROJECT IS UNIQUE
Do you have any questions? Send us a message and we will get in contact with you to find the best solution for your needs.
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