Transient Transfection

production of mammalian derived proteins and antibodies in weeks

The delivery of DNA into mammalian cells through non-viral methods with temporary effects is known as transient transfection. In the case of delivering functional coding DNA, the term “transient gene expression” (TGE) is widely used. TGE is a commonly used method for the production of recombinant proteins. Whilst commercial production of tons of biopharmaceuticals uses consistently productive stable cell lines (which take many months to develop), the production of milligram to gram amounts of high-grade material for early pre-clinical and biochemical characterization studies can be performed by TGE systems within the drug development pipeline. This website focuses on the production of recombinant proteins and antibodies using TGE. It will give you an overview of the main aspects and influencing factors.

Graphical overview of transient transfection

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