production of mammalian derived proteins and antibodies in weeks
The delivery of DNA into mammalian cells through non-viral methods with temporary effects is known as transient transfection. In the case of delivering functional coding DNA the term “transient gene expression” or for short TGE is widely used. TGE is a commonly used method to produce proteins. Whilst commercial production of tons of biopharmaceuticals uses consistently productive stable cell lines (which take many months to develop), the production of milligram to gram amounts of high-grade material for early pre-clinical and biochemical characterization studies can be performed by transient gene expression systems within the drug development pipeline. This website focuses on the production of recombinant proteins, antibodies and membrane proteins by TGE. It will give you an overview of the main aspects and influencing factors.
Graphical overview of transient transfection
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